Wang, N., Xie, Y.P., & Fan, J.H. Pathogenicity of Simplicillium lanosoniveum TYL001 isolated from Pseudaulacaspis pentagona.
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Notes: The entomopathogenic fungus Simplicillium lanosoniveum strain TYL001 was isolated from the naturally diseased Pseudaulacaspis pentagona collected from Fraxinus chinensis in Taiyuan, Shanxi Province of China, and identified based on morphological observation and molecular identification. The armored scale was inoculated with conidial suspension of the strain TYL001 in five concentrations (1.0×106conidia/mL, 5.0×106conidia/mL, 1.0×107conidia/mL, 5.0×107conidia/mL, 1.0×108conidia/mL). It was shown that after inoculation the infection rates of P. pentagona female adults reached 80.00%, 83.33%, 86.67%, 90.00%, and 93.33% respectively in six days with the five concentrations. It was indicated that the strain TYL001 has high infectivity against P. pentagona and shown that the infection rate increased with every increase of conidial concentration. The peak hours of infection appeared in 72–120h, and the higher conidial concentration the earlier symptom appearance. For the five conidial concentrations, the IT50 of the female adults was 106.95h, 97.00h, 91.46h, 87.73h, 79.38h, respectively, indicating that the infection priority was correlated with conidial concentration. Our study provides a new strain for biological control of Pseudaulacaspis pentagona.
