Zhao, X.P., Shu, C., Yang, F., Liu, K.W., & Yu, D. 2002 [Effects of metal ions and urea on alkaline phosphatase from Ericerus pela (Chavannes).]. Acta Entomologica Sinica 45(3): 318-322.

Notes: The effects of metal ions and urea on the alkaline phosphatase (AKP) activity from Ericerus pela (Chavannes) were surveyed. The enzyme was purified from the adult female. After each metal ion and urea had been mixed with the enzyme, the enzyme activity was determined. K super(+), Na super(+) and Li super(+) had not any effect on the enzyme activity. Ca super(2+), Ba super(2+), Mg super(2+), Mn super(2+), Co super(2+), Ni super(2+) activated the enzyme. Zn super(2+), Cu super(2+), Cd super(2+) and Pb super(2+) inhibited the enzyme. The Ca super(2+) was a more efficacious activator and the effect was classified as noncompetitive type. The inhibition of Cu super(2+) on the enzyme was found to be of noncompetitive type. The AKP was inactivated by urea; the inactivation could be divided into two kinds according to the concentration of the urea: lower than 3 mol/L and higher than 3 mol/L. At lower concentration, the effect of urea on the enzyme displayed a mixed type of inhibition.