Xi, Y., Yan, W., Liu, K., Cai, B., & Wu, S. 2024 Rapid Identification of Tropical Important Mealybugs Based on a Multiplex PCR Assay. Agronomy https://doi.org/10.3390/agronomy14122786
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Notes: In this study, a multiplex polymerase chain reaction (mPCR) assay based on the cytochrome c oxidase subunit I (COI) gene was successfully constructed for the rapid identification of mealybugs. The 5′ end COI gene fragments of 12 mealybug species were amplified and sequenced. Furthermore, an mPCR assay was established to identify three common mealybug species in Hainan, namely Dysmicoccus neobrevipes, Maconellicoccus hirsutus, and Paracoccus marginatus.
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