Ramasubramanian, T., Ramaraju, K., & Nirmala, R. 2016 COI Gene-based Species Diagnostic Kit for Sugarcane Scale Insect, Melanaspis glomerata (Green) (Homoptera: Diaspididae). Sugar Tech 18(4): 441-446

Keywords:

DNA sequencing

diagnosis

economic importance

Notes: A. COI gene-based DNA barcode was generated for sugarcane scale insect, Melanaspis glomerata (Green). The barcode developed (GenBank Accession Number KR153875) would probably be the first for this species. The extent of identity between the COI sequences of sugarcane scale and other three species of Diaspidids (Aonidiella aurantii, A. taxus and Melanaspis bromeliae) varied between 87.37 and 88.75 %. Therefore, the barcode generated in this study could easily delineate M. glomerata from other armoured scales of economic importance.