Palma-Jiménez, M., & Blanco-Meneses, M. 2015 Acondicionamiento de la Cochinilla Harinosa (Hemiptera: Pseudococcidae) Por Microscopía Electrónica de Barrido [Conditioning of mealybug (Hemiptera: Pseudococcidae) by Scanning Electron Microscopy.]. Agronomía Mesoamericana 26(2): 355-364

Keywords:

  • SEM
  • illustration
  • Notes: Four types of methodologies were implemented, which evidenced a gradual improvement of the observation of the ultrastructures through the Scanning Electron Microscopy. Every process was described in detail. The best results were showed with 10% xylene (in some cases it was feasible using 95-100% ethanol). It allowed to remove the wax from the body of the insect, avoiding its collapse, and observing the specific ultrastructures of the individual. This approach will reduce the time and cost of future taxonomic research of mealybugs.