Navarro, C.A., Von Bernath, D., Acevedo-Lopez, J., Mondaca, J., & Martinez-Bussenius, C. Molecular identification of mealybugs species (Hemiptera: Pseudococcidae) by qPCR melting curve analysis. Journal of Applied Entomology 0: 1-10
Keywords:
Notes: This study develops a more efficient and economical way to identify six Pseudococcidae species, including immature states and male adults that cannot be identified through traditional taxonomy. A qPCR melting curve analysis technique was used, and specific primers were developed for the ITS2 rDNA region, allowing the identification of all target species with 100% accuracy. The method also demonstrated potential for identifying additional mealybug species, making it valuable for phytosanitary surveillance. https://doi.org/10.1111/jen.13445
