Modafferi, A., Urbaneja, A., Aure, C.M., Laudani, F., Palmeri, V., Giunti, G., Campolo, O., & Pérez‑Hedo, M. 2025 Green pest control strategies: essential oil‑based nano‑emulsions for Delottococcus aberiae management. Journal of Pest Science 1-13
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Notes: This study aimed to develop stable and effective EO-based nano-emulsions with a high EO:surfactant ratio (3:1) through the high-pressure microfluidization technique. Firstly, the EOs (i.e., garlic, clove, and eucalyptus) and their nano-emulsions were chemically and physically characterized through gas chromatography-mass spectrometry (GC–MS) and dynamic light scattering (DLS) analyses, respectively. Then the EO-based nano-emulsions were used to investigate the biological activity against the second instars of D. aberiae, and the most efficacy nano-emulsion (i.e., garlic nano-emulsion) was subjected to further investigations in order to assess its toxicity on nontarget organisms such as C. montrouzieri and citrus plants. At the same time, the effects of garlic nano-emulsion on transcriptional levels of different genes involved in plant defense mechanisms were also investigated. https://doi.org/10.1007/s10340-025-01914-1