Latina, R.A., Lantican, D.V., Guerrero, M.S., Rubico, E.C., Laquinta, J.F., & Caoili, B.L. 2022 Species-specific PCR-based marker for rapid detection of Aspidiotus rigidus Reyne (Hemiptera: Diaspididae). Journal of Asia-Pacific Entomology 25 (2022) 101848

Keywords:

DNA

Notes: Molecular identification via PCR sequencing of insect barcoding genes has been implemented, but the overall process is time-consuming and costly. Thus, we developed and optimized a species-specific PCR-based molecular marker for rapid, efficient and cost-effective molecular identification of A. rigidus. The molecular marker was designed based on the sequences of the partial 28S ribosomal RNA gene from species of Aspidiotus that feed on coconut in the Philippines, A. rigidus, A. destructor and A. excisus.