Harish, E.R., & Aryalakshmi, M. 2022 Detection of specific endosymbionts of mealybugs infesting cassava (Manihot esculenta Crantz) using diagnostic PCR and Sanger sequencing. Journal of Tropical Agriculture


  • Wolbachia
  • endosymbionts
  • resistance
  • Notes: Mealybugs have been reported to be the main concern in cassava production for the past few years in all the major tuber cultivated ecosystems of India. Endosymbiotic bacteria found in mealybugs have been observed to interact and provide specific advantages to the host and they can be one of the reasons for the pests’ insecticidal resistance. In the present study, endosymbiotic bacteria were isolated from cassava mealybugs, allowed to grow in media after insecticidal treatments, and after observing colony morphology, distinct bacterial colonies were sub-cultured and selected for further molecular identification procedures.