Fajardo, T.V.M., Grynberg, P., Togawa, R.C., Fagundes Silva, J.M., da Silva, F.N., & Nickel, O. 2024 Analyzes of mealybug (Pseudococcus longispinus) virome reveal grapevine viruses diversity. Tropical Plant Pathology doi.org/10.1007/s40858-024-00647-3

Keywords:

vector

virus

Notes: Metagenomic analysis of nucleic acids extracted from insect vectors makes it possible to study the diversity of insect viruses in addition to plant pathogenic viruses. In this study, insects (Ps. longispinus) were collected, and pooled throughout a plot of virus disease symptomatic vines, cultivated in growing beds, and analyzed by high throughput sequencing (HTS). The complete genome of grapevine leafroll-associated virus 2 and 3 (GLRaV-2 and -3) and a partial sequence of grapevine virus A (GVA) with two complete ORFs (coat protein and RNA-binding protein) were assembled from mealybug extracts and exhibited high nucleotide identities, up to 99%, with previously characterized homologous Brazilian isolates from grapevines.