Chen, Y.-T., Shi, M.-Z., Chen, Y., Zhao, J.-W., Yang, X.-J., Fu, J.-W., Desneux, N., & Li, J.-Y. 2024 Rapid and equipment-free identification of papaya mealybug Paracoccus marginatus based on RPA-CRISPR/Cas12a. Pest Management Science https://doi.org/10.1002/ps.8425

Keywords:

DNA sequencing

control

molecular biology

Notes: This study presents a method that combines RPA and CRISPR/Cas12a with fluorescence and lateral flow technologies to differentiate P. marginatus from 10 other mealybugs. This RPA-CRISPR/Cas12a assay has the potential to detect P. marginatus with high accuracy in less than 1 h without the need for any experimental equipment. This provides a simple and convenient method that can be deployed in the field for detecting P. marginatus.

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