Amouroux, P., Crochard, D., Germain, J.-F., Correa, M.C.G., Ampuero, J., Groussier, G., Kreiter, P., Malausa, T., & Zaviezo, T. 2017 Genetic diversity of armored scales (Hemiptera: Diaspididae) and soft scales (Hemiptera: Coccidae) in Chile. Scientific Reports 7: 2014 | DOI:10.1038/s41598-017-01997-6

Keywords:

  • DNA
  • genebank
  • phylogenetics
  • phylogeny
  • Notes: We sequenced the COI and 28S genes in each population. In total, 19 Diaspididae species and 11 Coccidae species were identified morphologically. From the 63 COI haplotypes and the 54 28S haplotypes uncovered, and using several DNA data analysis methods (Automatic Barcode Gap Discovery, K2P distance, NJ trees), up to 36 genetic clusters were detected. Morphological and DNA data were congruent, except for three species (Aspidiotus nerii, Hemiberlesia rapax and Coccus hesperidum) in which DNA data revealed highly differentiated lineages. More than 50% of the haplotypes obtained had no high-scoring matches with any of the sequences in the GenBank database. This study provides 63 COI and 54 28S barcode sequences for the identification of Coccoidea from Chile.